Determination of Losartan Potassium and Perindopril Erbumine in Tablet Formulations by Reversed-Phase HPLC

The present work describes a validated reverse phase high performance liquid chromatographic method for simultaneous estimation of losartan potassium and Perindopril Erbumine in tablet formulation. Chromatography was performed on a ODS Hypersil C18 (250 mm x 4.6 mm i.d., 5 μm particle size) column with mobile phase containing Acetonitrile: Acidic Water pH 3.4 (50+50). The flow rate was 1.5 mL/min and the eluent was monitored at 218 nm. The selected chromatographic conditions were found to effectively separate Losartan Potassium (RT4.57 min) and Perindopril Erbumine (RT2.31 min). Linearity for losartan potassium and Perindopril Erbumine were found in the range of 1-30 μg/mL. The values obtained of LODs were 0.182 and 0.272 μg/mL, LOQs were 0.551 and 0.826 μg/mL for losartan potassium and Perindopril Erbumine, respectively. The proposed method was found to be fast, accurate, precise, and reproducible and can be used for simultaneous analysis of these drugs in tablet formulations.